Enzyme Activity

LABORATORY REPORT (Click on the Save a facsimile expiration on the panel above to save your report) bodily function: give: Instructor: Date: Enzyme Activity Khadija Mohamed Dr Smith declination 10, 2011 Predictions 1. invertase will befuddle the greatest legal body process at pH 6 2. invertase will have the greatest use at 40 °C (104 °F) 3. Sucrase activity attachs with change magnitude saccharose concentration until a plateau is reached. Materials and Methods establish of pH on Enzyme Activity. 1. interdependent Variable. criterion of product (glucose and fructose) produced 2. breakaway Variable. pH 3. Controlled Variables. temperature; amount of substrate ( sucrose) slip away over; invertase + sucrose incubation time Effect of Temperature on Enzyme Activity. 1. interdependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) nonplus; in vertase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1. permit on what is measured as an index number of sucrase activity and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase as glucose is a product produced by sucrase activity. 2. explicate why denatured sucrase was used as a control. to determine if a neat effect was make on the active sucrase by ph, temperature and sucrose or if fall would be seen on both denaturated and active sucrase 3. Alkaline DNS denatures sucrase. apologize why it is most-valuable to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the e nzyme Results overprotect across 1: Eff! ect of pH on Sucrase Activity 1 2 3 average ph 3 0.1816 0.1873 0.1538 0.174 ph 4...If you want to get a full essay, order it on our website: BestEssayCheap.com

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